In describing a protein it is now common to distinguish the primary, secondary and tertiary structures. The primary structure is simply the order, or sequence, of the amino-acid residues along the polypeptide chains. This was first determined by [Frederick] Sanger using chemical techniques for the protein insulin, and has since been elucidated for a number of peptides and, in part, for one or two other small proteins. The secondary structure is the type of folding, coiling or puckering adopted by the polypeptide chain: the a-helix structure and the pleated sheet are examples. Secondary structure has been assigned in broad outline to a number of librous proteins such as silk, keratin and collagen; but we are ignorant of the nature of the secondary structure of any globular protein. True, there is suggestive evidence, though as yet no proof, that a-helices occur in globular proteins, to an extent which is difficult to gauge quantitatively in any particular case. The tertiary structure is the way in which the folded or coiled polypeptide chains are disposed to form the protein molecule as a three-dimensional object, in space. The chemical and physical properties of a protein cannot be fully interpreted until all three levels of structure are understood, for these properties depend on the spatial relationships between the amino-acids, and these in turn depend on the tertiary and secondary structures as much as on the primary. Only X-ray diffraction methods seem capable, even in principle, of unravelling the tertiary and secondary structures.[Co-author with G. Bodo, H. M. Dintzis, R. G. Parrish, H. Wyckoff, and D. C. Phillips]
All at once, something wonderful happened, although at first, it seemed perfectly ordinary. A female goldfinch suddenly hove into view. She lighted weightlessly on the head of a bankside purple thistle and began emptying the seedcase, sowing the air with down.tThe lighted frame of my window filled. The down rose and spread in all directions, wafting over the dam’s waterfall and wavering between the tulip trunks and into the meadow. It vaulted towards the orchard in a puff; it hovered over the ripening pawpaw fruit and staggered up the steep faced terrace. It jerked, floated, rolled, veered, swayed. The thistle down faltered down toward the cottage and gusted clear to the woods; it rose and entered the shaggy arms of pecans. At last it strayed like snow, blind and sweet, into the pool of the creek upstream, and into the race of the creek over rocks down. It shuddered onto the tips of growing grasses, where it poised, light, still wracked by errant quivers. I was holding my breath. Is this where we live, I thought, in this place in this moment, with the air so light and wild?tThe same fixity that collapses stars and drives the mantis to devour her mate eased these creatures together before my eyes: the thick adept bill of the goldfinch, and the feathery coded down. How could anything be amiss? If I myself were lighter and frayed, I could ride these small winds, too, taking my chances, for the pleasure of being so purely played.tThe thistle is part of Adam’s curse. “Cursed is the ground for thy sake, in sorrow shalt thou eat of it; thorns also and thistles shall it bring forth to thee.” A terrible curse: But does the goldfinch eat thorny sorrow with the thistle or do I? If this furling air is fallen, then the fall was happy indeed. If this creekside garden is sorrow, then I seek martyrdom. tI was weightless; my bones were taut skins blown with buoyant gas; it seemed that if I inhaled too deeply, my shoulders and head would waft off. Alleluia.